Question 1

In the case of genetic exchange in bacteria by transformation, there is an exchange of DNA back and forth between cells, and a complete diploid cell is formed.

Accepted Answer

A) True 
 B)False

Question 2

How would you design an experiment to test whether a transduction event occurred between two different bacterial strains?

Accepted Answer

You would use a selected marker to ident...

Question 3

During a cis-trans complementation assay, two lac- strains of bacteria are crossed. The progeny cells exhibit a lac+ phenotype. What does this indicate?

Accepted Answer

The two mutations are in diffe...

Question 4

To grow on artificial medium, prototrophic bacteria require

Accepted Answer

A)   amino acid supplements.
B)   vitamin supplements.
C)   sunlight.
D)   all of the above.
E)   none of the above.F) A) and B)
G) A) and C)

Question 5

How could you use T2 bacteriophage plaque phenotypes to determine the genetic distance between two genes, h and r, affecting plaque appearance in the phage DNA?

Accepted Answer

Just as in eukaryotic organisms, a cross...

Question 6

Cotransductant bacteria can occur if

Accepted Answer

A)   a bacterial cell picks up two fragments of DNA from the environment, each with a different gene.
B)   two genes are closely linked enough so that they can be packaged into a phage head and injected into a cell by a single phage.
C)   double-crossover occurs between prophage DNA and the bacterial chromosome.
D)   two genes are introduced into the same bacterium by simultaneous infection with two different phages.
E)   Both C and DF) A) and E)
G) A) and B)

Question 7

How did Seymour Benzer use deletion mapping to construct fine-scale maps of the rII region of the T4 bacteriophage genome?

Accepted Answer

He used deletion mutants, which, unlike ...

Question 8

All the cells in a bacterial colony are genetically identical.

Accepted Answer

A) True 
 B)False

Question 9

Which type of genetic exchange in bacteria involves a plasmid?

Accepted Answer

A)   Conjugation
B)   Transduction
C)   Transformation
D)   Both A and C
E)   All of the aboveF) A) and D)
G) A) and C)

Question 10

Bacillus subtilis is an example of a bacteria that must be engineered to undergo genetic transformation.

Accepted Answer

A) True 
 B)False  False

Question 11

Genetic mapping experiments performed by Seymour Benzer on rII mutants in the T4 phage showed that the gene is indivisible by the process of mutation and recombination.

Accepted Answer

A) True 
 B)False

Question 12

In general, transformation of most genes occurs at a frequency of one in every ________ cells.

Accepted Answer

A)   10
B)   100
C)   1,000
D)   10,000
E)   1,000,000F) A) and C)
G) B) and D)

Question 13

What are temperate bacteriophages?

Accepted Answer

Phages that can alte...

Question 14

A bacterial broth culture is diluted by a factor of 100,000, and 0.1 mL of the dilution is spread over the surface of an agar plate. The next day, 123 colonies are observed on the plate. What was the concentration of bacteria in the broth?

Accepted Answer

We want to determine the number of colony forming units (cfu) per mL. Since 123 colonies are observed on the plate, this means that there were 123 bacterial cells (cfu) in 0.1 mL of the 100,000X dilution. Thus, in the original culture, there were 123 cfu × 100,000 (dilution factor) × 10 = 1.23 × 108 cfu/mL.

Question 15

In the lysogenic bacteriophage life cycle, the λ chromosome

Accepted Answer

A)   replicates and the phage genes take over the bacterium.
B)   inserts itself physically into the host cell's chromosome.
C)   expresses a repressor protein gene that inhibits the lytic pathway.
D)   Both B and C
E)   is replaced by a piece of bacterial DNA when packaged inside phage progeny.F) A) and B)
G) A) and C)

Question 16

An arg- strain of E. coli is transformed with a plasmid carrying the wild-type (arg+)  gene. The transformed cells are replica-plated to two minimal medium plates, one supplemented with arginine and one without. The transformed cells would grow on

Accepted Answer

A)   only the plate lacking arginine.
B)   only the plate supplemented with arginine.
C)   both plates.
D)   neither plate.
E)   the plate supplemented with arginine only if a mutation occurred.F) A) and C)
G) B) and C) C

Question 17

In F+ × F- crosses, none of the bacterial chromosome is transferred; only the F factor is.

Accepted Answer

A) True 
 B)False

Question 18

In a series of cotransformation experiments, DNA fragments from x+y+z+ donor bacteria were used to transform xyz recipient bacteria. The following transformation phenotypes were observed in the recipient cells: xy+z xyz+ x+y+z x+yz+ What does this imply about the gene order of x, y, and z?

Accepted Answer

The gene order must be y-x-z. Since the ...

Question 19

The viral chromosome of a bacteriophage can never integrate into the host bacterium's chromosome.

Accepted Answer

A) True 
 B)False

Question 20

________ bacterial strains require nutritional supplements in order to grow in a minimal medium.

Accepted Answer

A)   Autotrophic
B)   Auxotrophic
C)   Prototrophic
D)   Phototrophic
E)   HeterotrophicF) A) and B)
G) A) and D)

Exam 25: Genetics: an Introduction

In the case of genetic exchange in bacteria by transformation, there is an exchange of DNA back and forth between cells, and a complete diploid cell is formed.

Correct Answer
verified

How would you design an experiment to test whether a transduction event occurred between two different bacterial strains?

Correct Answer
verified
You would use a selected marker to ident...
View Answer

During a cis-trans complementation assay, two lac- strains of bacteria are crossed. The progeny cells exhibit a lac+ phenotype. What does this indicate?

Correct Answer
verified
The two mutations are in diffe...
View Answer

To grow on artificial medium, prototrophic bacteria require

Correct Answer
verified

How could you use T2 bacteriophage plaque phenotypes to determine the genetic distance between two genes, h and r, affecting plaque appearance in the phage DNA?

Correct Answer
verified
Just as in eukaryotic organisms, a cross...
View Answer

Cotransductant bacteria can occur if

Correct Answer
verified

How did Seymour Benzer use deletion mapping to construct fine-scale maps of the rII region of the T4 bacteriophage genome?

Correct Answer
verified
He used deletion mutants, which, unlike ...
View Answer

All the cells in a bacterial colony are genetically identical.

Correct Answer
verified

Which type of genetic exchange in bacteria involves a plasmid?

Correct Answer
verified

Bacillus subtilis is an example of a bacteria that must be engineered to undergo genetic transformation.

Correct Answer
verified
False

Genetic mapping experiments performed by Seymour Benzer on rII mutants in the T4 phage showed that the gene is indivisible by the process of mutation and recombination.

Correct Answer
verified

In general, transformation of most genes occurs at a frequency of one in every ________ cells.

Correct Answer
verified

What are temperate bacteriophages?

Correct Answer
verified
Phages that can alte...
View Answer

A bacterial broth culture is diluted by a factor of 100,000, and 0.1 mL of the dilution is spread over the surface of an agar plate. The next day, 123 colonies are observed on the plate. What was the concentration of bacteria in the broth?

In the lysogenic bacteriophage life cycle, the λ chromosome

Correct Answer
verified

An arg- strain of E. coli is transformed with a plasmid carrying the wild-type (arg+) gene. The transformed cells are replica-plated to two minimal medium plates, one supplemented with arginine and one without. The transformed cells would grow on

Correct Answer
verified
C

In F+ × F- crosses, none of the bacterial chromosome is transferred; only the F factor is.

Correct Answer
verified

In a series of cotransformation experiments, DNA fragments from x+y+z+ donor bacteria were used to transform xyz recipient bacteria. The following transformation phenotypes were observed in the recipient cells: xy+z xyz+ x+y+z x+yz+ What does this imply about the gene order of x, y, and z?

Correct Answer
verified
The gene order must be y-x-z. Since the ...
View Answer

The viral chromosome of a bacteriophage can never integrate into the host bacterium's chromosome.

Correct Answer
verified

________ bacterial strains require nutritional supplements in order to grow in a minimal medium.

Correct Answer
verified

Exam 25: Genetics: an Introduction

In the case of genetic exchange in bacteria by transformation, there is an exchange of DNA back and forth between cells, and a complete diploid cell is formed.

Correct AnswerverifiedShow Answer

How would you design an experiment to test whether a transduction event occurred between two different bacterial strains?

Correct AnswerverifiedYou would use a selected marker to ident...View AnswerShow Answer

During a cis-trans complementation assay, two lac- strains of bacteria are crossed. The progeny cells exhibit a lac+ phenotype. What does this indicate?

Correct AnswerverifiedThe two mutations are in diffe...View AnswerShow Answer

To grow on artificial medium, prototrophic bacteria require

Correct AnswerverifiedShow Answer

How could you use T2 bacteriophage plaque phenotypes to determine the genetic distance between two genes, h and r, affecting plaque appearance in the phage DNA?

Correct AnswerverifiedJust as in eukaryotic organisms, a cross...View AnswerShow Answer

Cotransductant bacteria can occur if

Correct AnswerverifiedShow Answer

How did Seymour Benzer use deletion mapping to construct fine-scale maps of the rII region of the T4 bacteriophage genome?

Correct AnswerverifiedHe used deletion mutants, which, unlike ...View AnswerShow Answer

All the cells in a bacterial colony are genetically identical.

Correct AnswerverifiedShow Answer

Which type of genetic exchange in bacteria involves a plasmid?

Correct AnswerverifiedShow Answer

Bacillus subtilis is an example of a bacteria that must be engineered to undergo genetic transformation.

Correct AnswerverifiedFalse

Genetic mapping experiments performed by Seymour Benzer on rII mutants in the T4 phage showed that the gene is indivisible by the process of mutation and recombination.

Correct AnswerverifiedShow Answer

In general, transformation of most genes occurs at a frequency of one in every ________ cells.

Correct AnswerverifiedShow Answer

What are temperate bacteriophages?

Correct AnswerverifiedPhages that can alte...View AnswerShow Answer

A bacterial broth culture is diluted by a factor of 100,000, and 0.1 mL of the dilution is spread over the surface of an agar plate. The next day, 123 colonies are observed on the plate. What was the concentration of bacteria in the broth?

In the lysogenic bacteriophage life cycle, the λ chromosome

Correct AnswerverifiedShow Answer

An arg- strain of E. coli is transformed with a plasmid carrying the wild-type (arg+) gene. The transformed cells are replica-plated to two minimal medium plates, one supplemented with arginine and one without. The transformed cells would grow on

Correct AnswerverifiedC

In F+ × F- crosses, none of the bacterial chromosome is transferred; only the F factor is.

Correct AnswerverifiedShow Answer

In a series of cotransformation experiments, DNA fragments from x+y+z+ donor bacteria were used to transform xyz recipient bacteria. The following transformation phenotypes were observed in the recipient cells: xy+z xyz+ x+y+z x+yz+ What does this imply about the gene order of x, y, and z?

Correct AnswerverifiedThe gene order must be y-x-z. Since the ...View AnswerShow Answer

The viral chromosome of a bacteriophage can never integrate into the host bacterium's chromosome.

Correct AnswerverifiedShow Answer

________ bacterial strains require nutritional supplements in order to grow in a minimal medium.

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
You would use a selected marker to ident...
View Answer

Correct Answer
verified
The two mutations are in diffe...
View Answer

Correct Answer
verified

Correct Answer
verified
Just as in eukaryotic organisms, a cross...
View Answer

Correct Answer
verified

Correct Answer
verified
He used deletion mutants, which, unlike ...
View Answer

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
False

Correct Answer
verified

Correct Answer
verified

Correct Answer
verified
Phages that can alte...
View Answer

Correct Answer
verified

Correct Answer
verified
C

Correct Answer
verified

Correct Answer
verified
The gene order must be y-x-z. Since the ...
View Answer

Correct Answer
verified

Correct Answer
verified