Question 1

Which of the following microbes produces a protein that kills a variety of insect pests?

Accepted Answer

A)   Pseudomonas
B)   Plasmodium falciparum
C)   Haemophilus influenzae
D)   Bacillus thuringiensis
E)   Thermus aquaticusF) B) and E)
G) A) and B)

Question 2

A set of clones representing the entire genome of an organism is known as a gene (recombinant/map/library).

Accepted Answer

The answer of A set of clones representing the entire...

Question 3

Probes used for detecting genetic sequences are frequently composed of

Accepted Answer

A)   synthetic nucleic acids and labeled conjugates, such as fluorescent dyes.
B)   restriction enzymes.
C)   nitrocellulose.
D)   agarose.
E)   silicon chips.F) None of the above
G) C) and D)

Question 4

Which of the following devices is used for PCR?

Accepted Answer

A)   a gene gun
B)   a nucleic acid synthesis machine
C)   a DNA sequencer
D)   an electrophoresis chamber
E)   a thermocyclerF) A) and B)
G) A) and C)

Question 5

Under ideal conditions, the number of DNA molecules produced during PCR increases exponentially.

Accepted Answer

A) True 
 B)False

Question 6

The process of locating genes within the nucleic acid of an organism is called (genetic/library/clone) mapping.

Accepted Answer

The answer of The process of locating genes within the...

Question 7

Some of the strongest opposition to the application of recombinant DNA technology concerns

Accepted Answer

A)   DNA fingerprinting.
B)   correcting gene defects in animals.
C)   modification of food crops.
D)   sequencing of the human genome.
E)   pest control measures.F) B) and D)
G) A) and D)

Question 8

Vectors usually contain genetic (markers/sequences) such as antibiotic genes or fluorescent tags.

Accepted Answer

The answer of Vectors usually contain genetic (markers/sequences) such as...

Question 9

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel (electroporation/electrophoresis).

Accepted Answer

The answer of The northern blot is a technique used...

Question 10

Compare and contrast the two major categories of restriction enzymes.

Accepted Answer

All restriction enzymes recognize specif...

Question 11

are used in the detection of DNA by FISH.

Accepted Answer

A)   Restriction fragments
B)   Compressed air and gold beads
C)   Reverse transcriptase and DNA polymerase
D)   DNA polymerase and DNA ligase
E)   Synthetic DNAs and fluorescent tagsF) D) and E)
G) None of the above

Question 12

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

Accepted Answer

A)   32
B)   16
C)   100
D)   6
E)   64F) All of the above
G) None of the above

Question 13

The use of microbes to make practical products such as vaccines or hormones is called (genomics/recombination/biotechnology).

Accepted Answer

The answer of The use of microbes to make practical...

Question 14

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/primers/vectors).

Accepted Answer

The answer of Short nucleic acid molecules used to locate...

Question 15

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?

Accepted Answer

A)   use a microarray to detect a transcript of the gene
B)   use a labeled synthetic probe complementary to the gene sequence
C)   use electrophoresis to identify plasmids containing an insert of the expected size
D)   sequence the DNA of the plasmids from each isolate
E)   assay for activity of the gene productF) A) and E)
G) B) and E)

Exam 8: Recombinant Dna Technology

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel (electroporation/electrophoresis).

Correct Answer
verified

Compare and contrast the two major categories of restriction enzymes.

Correct Answer
verified
All restriction enzymes recognize specif...
View Answer

Which of the following devices is used for PCR?

Correct Answer
verified

are used in the detection of DNA by FISH.

Correct Answer
verified

The process of locating genes within the nucleic acid of an organism is called (genetic/library/clone) mapping.

Correct Answer
verified

Some of the strongest opposition to the application of recombinant DNA technology concerns

Correct Answer
verified

Which of the following microbes produces a protein that kills a variety of insect pests?

Correct Answer
verified

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?

Correct Answer
verified

Under ideal conditions, the number of DNA molecules produced during PCR increases exponentially.

Correct Answer
verified

The use of microbes to make practical products such as vaccines or hormones is called (genomics/recombination/biotechnology).

Correct Answer
verified

A set of clones representing the entire genome of an organism is known as a gene (recombinant/map/library).

Correct Answer
verified

Vectors usually contain genetic (markers/sequences) such as antibiotic genes or fluorescent tags.

Correct Answer
verified

Probes used for detecting genetic sequences are frequently composed of

Correct Answer
verified

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/primers/vectors).

Correct Answer
verified

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

Correct Answer
verified

Exam 8: Recombinant Dna Technology

The northern blot is a technique used to detect specific RNA molecules in a larger population of molecules that have been separated by gel (electroporation/electrophoresis).

Correct AnswerverifiedShow Answer

Compare and contrast the two major categories of restriction enzymes.

Correct AnswerverifiedAll restriction enzymes recognize specif...View AnswerShow Answer

Which of the following devices is used for PCR?

Correct AnswerverifiedShow Answer

are used in the detection of DNA by FISH.

Correct AnswerverifiedShow Answer

The process of locating genes within the nucleic acid of an organism is called (genetic/library/clone) mapping.

Correct AnswerverifiedShow Answer

Some of the strongest opposition to the application of recombinant DNA technology concerns

Correct AnswerverifiedShow Answer

Which of the following microbes produces a protein that kills a variety of insect pests?

Correct AnswerverifiedShow Answer

A researcher inserted DNA fragments from an organism into plasmids and introduced the modified plasmids into bacterial cells. Which of the following methods would be the most efficient means of identifying which clones contain a specific gene of interest?

Correct AnswerverifiedShow Answer

Under ideal conditions, the number of DNA molecules produced during PCR increases exponentially.

Correct AnswerverifiedShow Answer

The use of microbes to make practical products such as vaccines or hormones is called (genomics/recombination/biotechnology).

Correct AnswerverifiedShow Answer

A set of clones representing the entire genome of an organism is known as a gene (recombinant/map/library).

Correct AnswerverifiedShow Answer

Vectors usually contain genetic (markers/sequences) such as antibiotic genes or fluorescent tags.

Correct AnswerverifiedShow Answer

Probes used for detecting genetic sequences are frequently composed of

Correct AnswerverifiedShow Answer

Short nucleic acid molecules used to locate complementary sequences in a larger population of molecules are called (probes/primers/vectors).

Correct AnswerverifiedShow Answer

If you started with a single DNA molecule, how many would you have at the end of six PCR cycles?

Correct AnswerverifiedShow Answer

Correct Answer
verified

Correct Answer
verified
All restriction enzymes recognize specif...
View Answer

Correct Answer
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